切磋开采人多能干细胞肝向不同的第贰调节因子，关于举办美利坚联邦合众国国立卫生商量院

告诉标题：Induced pluripotent stem cells from a hibernator: how studying
hibernation in a dish may benefit us

报告标题：Induced pluripotent stem cells from a hibernator: how studying
hibernation in a dish may benefit us

7月三十一日，国际学术期刊Stem Cell Reports
在线发布了中国中国科学技术大学学香港（Hong Kong）血红蛋白与健康研究院丁秋蓉与邵振研讨组的合营研究成果Genetic
and chemical screenings identify HDAC3 as a key regulator in hepatic
differentiation of human pluripotent stem
cells。该研讨通过广泛遗传筛选和化合物筛选判别出包罗组蛋白去乙酰化酶HDAC叁等在内的人多能干细胞（human
pluripotent stem cells, hPSCs）体外肝向差距关键调整因子。

“目录号: HY-14650

报 告 人：区景行 商量员（美利坚合众国国立卫生研究院）

报 告 人：区景行 研究员（NIH）

人多能干细胞体外定向分化来源的肝脏样细胞(hepatocyte like cells,
HLCs)是肝脏疾病模拟、毒理检查测试以及肝脏移植供体的暧昧主要细胞来源。但当下拿走的HLCs广泛存在成熟度不够、纯度不够的主题材料，限制了其在基础科学和转载艺术学方面包车型客车利用。

Others-

报告时间：二零一八年三月230日（星期4）中午10:30-1一:30

告诉时间：201八年7月二二十三日十:00-1一:30

为越发优化体外定向差异体系，促进肝脏细胞的老道和均一化，博士大学生陈佩华等人在肝脏成熟标识物白蛋白(ALB)终止密码子前一定敲入蓝灰荧光蛋白表达框，营造了成熟肝脏谱系标志的人多能干细胞报告株。并在此基础上，运用C福睿斯ISPXC60全基因组敲除慢病毒文库，进行了大规模遗传筛选，推断出ATG7,RPS6KA2,
HDAC3
等若干基因参与调治将养人多能干细胞体外肝向不相同。同时依附筛选出的调治基因及其有关功用提醒，进一步张开了有指向的化合物文库筛选，发掘三个组蛋白去乙酰化酶小分子抑制剂CI-994能精通促进HLCs的定向差别；并开采该小分子同样能平抑原代肝脏细胞体外作育进程中肝脏属性的飞速丢失。全基因组敲除慢病毒文库筛选和小分子化合物库筛选都表明了组蛋白去乙酰化酶HDAC三是肝脏分裂的第3调节因子，进一步的研究开掘HDAC三方可与肝脏谱系关键转录因子HNF肆等组合共同调治肝脏细胞差异进度。

DHEA 是雄激素驷比不上舌根源，是一种有效的抗细胞凋亡因子。

告诉地方：高校城校区，生物科学与工程高校 B6-20七

告诉地方：食物科学与工程大学东糖厅

该商量综合使用报告基因细胞株、大规模遗传筛选以及化合物筛选，创建了3个非常的慢的体外细胞实验类别，用于研究人多能干细胞的肝脏细胞体外定向分歧进度，也为商量和优化hPSCs向别的门类的细胞分歧提供了一种普适而卓有功用的法子。

Androgen
Receptor

应接广大师生参预。

招待广大师生到场。

该钻探由杨凡、研讨助理李木山等在研讨员丁秋蓉、邵振和博士赵永旭的指导下完了，也获取了上海糖类与健康商讨院斟酌员应浩、方靖和王慧女士，美利坚合众国加州圣地亚哥分校学院解说KiranMusunuru，广州理经济高校助教马丹军的帮扶。同时，该研讨得到了国家主要研究开发安排、中国科高校干细胞早先、国家自然科学基金委员会等的同步帮助以及东方之珠营养与健康院公共才能平台的支撑。

有关制品

生物科学与工程高校

食物科学与工程高校

舆论链接

Enzalutamide-Dihydrotestosterone-Testosterone-切磋开采人多能干细胞肝向不同的第贰调节因子，关于举办美利坚联邦合众国国立卫生商量院。ARN-509-Bicalutamide-ODM-201-ASC-J9-MK-0773-Spironolactone-AZD3514-Flutamide-LGD-4033-3,3′-Diindolylmethane-GLPG0492-Danazol-

2018年4月24日

2018年4月17日

生物活性

告知摘录：In this lecture, Researcher JingXing Ou will show you the
secret of how the hibernator resist low temperature. Researchers of
National Institutes of Health first prepare the induced pluripotent stem
cells from a kind of hibernator, thirteen striped ground squirrels.
Comparing the difference of nerve cell between human and hibernator at
low temperature, clarifying the key mechanism of how the hibernator cell
resist to low temperature. After treating the nerve cell of human, it
can effectively maintain the cell morphology at low temperature and let
the mouse kidney resist the low temperature. In the future, scientists
will enable human beings to acquire the ability to resist extreme cold
and prolong life.

告诉摘要：

探讨开掘人多能干细胞肝向区别的首要调节因子

Description

报告人简要介绍：区景行，华工生物工程专门的工作玖7级校友，二零零六年大学生完成学业于大不列颠及北爱尔兰联合王国那格浦尔高校，主修细胞与发育生物学。二零一零-贰零1壹，负责U.S.国营神经病学与胸膜炎脑瘤探讨所（NINDS）访问学者；二〇一一-2014，担当U.S.A.国立性传播疾病应用切磋究所（NEI）访问学者；201肆现今，担负美利坚联邦合众国国立卫生钻探院（NIH）商量员。近年来已在国外著名杂志发布随想20余篇，并屡次在国际会议上发布主旨阐述。其商讨成果于近日在列国著名学术期刊《Cell》上线：NIH的物医学家利用第一回制备出的冬眠动物诱导多能干细胞，申明了细胞抵抗低温的建制，并对人神经细胞举办拍卖使其能够行得通地在低温维持细胞形态以及让小鼠的肾脏抵御低温。以后，人类抵御严寒并延伸寿命的冀望极有极大可能率落成。

In this lecture, Researcher JingXing Ou will show you the secret of how
the hibernator resist low temperature. Researchers of National
Institutes of Health first prepare the induced pluripotent stem cells
from a kind of hibernator, thirteen striped ground squirrels. Comparing
the difference of nerve cell between human and hibernator at low
temperature, clarifying the key mechanism of how the hibernator cell
resist to low temperature. After treating thenerve cell of human, it can
effectively maintain the cell morphology at low temperature and let the
mouse kidney resist the low temperature. In the future, scientists will
enable human beings to acquire the ability to resist extreme cold and
prolong life.

DHEA is an important source ofandrogens, and is an effective
antiapoptotic factor.

附件：无

报告人简要介绍：

IC50& Target

区景行，2010年学士毕业于英帝国福冈大学，主修细胞与发育生物学。2010-2011，担任美利坚合众国国立神经病学与偏高烧研商所（NINDS）访问学者；二零一一-2014，担负美利坚联邦合众国国立五官调研讨所（NEI）访问学者；201四到现在，担负U.S.A.国立卫生商量院（NIH）切磋员。近期已在外国资深杂志公布杂谈20余篇，并壹再在国际会议上刊载主旨发言。其研讨成果于目前在列国有名学术期刊《Cell》上线：NIH的物管理学家利用第三遍制备出的冬眠动物诱导多能干细胞，申明了细胞抵抗低温的建制，并对人神经细胞实行拍卖使其能够使得地在低温维持细胞形态以及让小鼠的肾脏抵御低温。现在，人类抵御严寒并拉开寿命的指望极有非常大希望达成。

Androgen
receptor[1];)

附件：无

In Vitro

DHEA is an effective antiapoptotic factor, reversing the serum
deprivation-induced apoptosis in prostate cancer cells (DU145 and LNCaP
cell lines) as well as in colon cancer cells (Caco2 cell line). DHEA
significantly reduces serum deprivation-induced apoptosis in all 3
cancer cell types, quantitated with the APOPercentage assay (apoptosis
is reduced from 0.587±0.053 to 0.142±0.0016 or 0.059±0.002 after
treatment for 12 hours with DHEA or NGF, respectively; n=3, P<0.01),
and by flow cytometry analysis (FACS) for DU145 cells. The antiapoptotic
effect of DHEA is dose dependent with an EC50 at nanomolar
concentrations (EC50: 11.2±3.6 nM and 12.4±2.2 nM in DU145 and Caco2
cells,
respectively)[1];).
DHEA is the principal sex steroid precursor in humans and can be
converted directly to androgens. DHEA (≥1 μM) causes a dose-dependent
inhibition of Chub-S7 proliferation, as assessed by thymidine
incorporation assays. DHEA treatment inhibits expression of the key
glucocorticoid-regulating genesH6PDH(≥100 nM) andHSD11B1(≥1 μM) in
differentiating preadipocytes in a dose-dependent manner. In keeping
with this finding, DHEA treatment (≥1 μM) results in a marked reduction
in 11β-HSD1 oxoreductase activity (≥1 μM) and a concurrent increase in
dehydrogenase activity at the highest DHEA dose used (25 μM DHEA) in
differentiated
adipocytes[2];).

In Vivo

DHEA in the diet (0.45 % w/w) of male B6 mice (groups of five mice)
treated for 8 weeks led to significant decreases in body temperature
compared with mice fed the control AIN-76A diet. A similar comparison
indicated that control and pair-fed mice are also significantly
different. Animals fed DHEA have significantly lower temperatures than
mice fed the control diet 26/29 times tested; mice pair fed to those on
the DHEA diet are less affected, with 8/29 values significantly lower
than in mice fed AIN-76Aad libitum. The temperatures of mice fed DHEA
or pair fed to DHEA are significantly different 21/29 times tested. Body
weights are significantly greater in mice fed the control diet than in
mice fed DHEA or pair fed to DHEA. Food intake (grams per day) from
cages are averaged for each week (n=7), except for Week 9 (n=3). The
amount of food intake is significantly decreased in mice fed DHEA. By
design, mice pair fed to DHEA ate about the same amount. Thus, it
appears that DHEA reduces body temperature by food restriction and by a
separate
mechanism[3];).

Clinical Trial

NCT02357472

ShangHai Ji Ai Genetics & IVF Institute

Infertility

December 2014

Phase 4

NCT02150330

Taipei Veterans General Hospital, Taiwan-National YangMing University

Dehydroepiandrosterone-DHEAS-Gene Expression of Cumulus Cells.-Ovarian
Hyper-stimulation Protocol.-Artificial Reproduction Technology.

January 2013

Phase 3

NCT00575341

University Hospital Tuebingen

Adrenal Insufficiency

October 2003

Phase 3

NCT00289926

Monash University

Quality of Life-Menopausal Syndrome-Libido Disorder

February 2006

Phase 3

NCT00900900

Jed E. Rose-Duke University

Substance Withdrawal Syndrome

July 2009

Phase 2

NCT00650754

Center for Human Reproduction

Primary Ovarian Insufficiency-Unexplained Infertility

March 2008

Phase 2-Phase 3

NCT02432248

The Affiliated Hospital of Inner Mongolia Medical University-First
Affiliated Hospital, Sun Yat-Sen University-Renmin Hospital of Wuhan
University

Subfertility

February 2015

NCT00948857

David H. Barad-Center for Human Reproduction

Premature Ovarian Failure

June 2009

Phase 2-Phase 3

NCT01861847

Humanetics Corporation

PTSD

February 2013

Phase 2

NCT00419913

Center for Human Reproduction-Foundation for Reproductive Medicine

Infertility-Decreased Ovarian Reserve

January 2007

Phase 2-Phase 3

NCT00916396

Tel-Aviv Sourasky Medical Center

Hypoactive Sexual Desire Disorder

June 2005

NCT01535872

KK Women’s and Children’s Hospital

Infertility-Poor Responder to IVF Treatment

February 2012

Phase 3

NCT00248989

CHU de Quebec-Universite Laval

Skin Aging-Quality of Life

November 1, 2004

Phase 3

NCT01129830

Virginia Center for Reproductive Medicine

Infertility

January 2010

Early Phase 1

NCT00391924

UMC Utrecht-University Medical Center Groningen-Dutch Arthritis
Association

Lupus Erythematosus, Systemic-Sjogren’s Syndrome

May 2000

Phase 2

NCT01572025

University of Nottingham

Infertility-Ovarian Aging-Diminished Ovarian Reserve (DOR)-Predicted
Poor-responders

May 2012

Phase 3

NCT00167609

University of Versailles-Association Française contre les Myopathies
(AFM), Paris-Assistance Publique – Hôpitaux de Paris

Myotonic Dystrophy

November 2004

Phase 2-Phase 3

NCT00001598

National Institute of Dental and Craniofacial Research (NIDCR)-National
Institutes of Health Clinical Center (CC)

Lacrimal Apparatus Disease-Salivary Gland Disease-Sjogren’s
Syndrome-Xerostomia

May 1997

Phase 2

NCT00189124

University of Michigan-Arthritis Foundation

Systemic Lupus Erythematosus

September 2003

Phase 2-Phase 3

NCT01145144

Meir Medical Center

Ovarian Stimulation

January 2008

NCT00543166

Helsinki University-Göteborg University-Uppsala University

Sjogren’s Syndrome

February 2003

Phase 4

NCT00607646

University of Maryland-National Institute of Diabetes and Digestive and
Kidney Diseases (NIDDK)

Type 1 Diabetes

October 2010

Early Phase 1

NCT00442403

Université Victor Segalen Bordeaux 2

Malaria

April 2002

Phase 3

NCT00471900

University of Aarhus

Adrenal Insufficiency

October 2001

NCT00001487

National Institute of Mental Health (NIMH)-National Institutes of Health
Clinical Center (CC)

Depressive Disorder-Mood Disorder

June 1995

Phase 2

NCT00566384

Bayer

Libido

November 2007

Phase 2

NCT00004795

National Center for Research Resources (NCRR)-Northwestern
University-Office of Rare Diseases (ORD)

Systemic Lupus Erythematosus

August 1994

Phase 2-Phase 3

NCT00004665

National Center for Research Resources (NCRR)-Northwestern
University-Office of Rare Diseases (ORD)

Systemic Lupus Erythematosus

June 1995

Phase 2

NCT00004662

National Center for Research Resources (NCRR)-Northwestern
University-Office of Rare Diseases (ORD)

Systemic Lupus Erythematosus

March 1996

Phase 3

NCT00004313

National Center for Research Resources (NCRR)-University of California,
Los Angeles-Office of Rare Diseases (ORD)

Addison’s Disease

August 1995

Phase 3

NCT02794948

Guangzhou University of Traditional Chinese Medicine

Primary Ovarian Insufficiency

April 2016

Early Phase 1

NCT01753037

Mayo Clinic

Hyperandrogenism

December 2006

NCT00310791

Boston Children’s Hospital-United States Department of Defense-Eunice
Kennedy Shriver National Institute of Child Health and Human Development
(NICHD)

Anorexia Nervosa

April 2004

Phase 2-Phase 3

NCT00106314

Inflabloc Pharmaceuticals

Crohn’s Disease

January 2005

Phase 2

NCT00673309

The University of Texas Medical Branch, Galveston-National Institutes of
Health (NIH)

Burns

July 2002

Phase 2-Phase 3

NCT02013544

EndoCeutics Inc.

Vaginal Atrophy

February 2014

Phase 3

NCT00082511

威尼斯人开户，Genelabs Technologies

Systemic Lupus Erythematosus

July 2003

Phase 3

NCT01376349

Alliance for Clinical Trials in Oncology-National Cancer Institute
(NCI)-Mayo Clinic

Breast Cancer-Gynecologic Cancer

July 2011

Phase 3

NCT00053560

Genelabs Technologies

Lupus

December 2002

Phase 3

NCT00006219

Mayo Clinic-National Cancer Institute (NCI)

Multiple Myeloma and Plasma Cell Neoplasm

August 2000

Phase 2

NCT01343771

Boston Children’s Hospital

Anorexia Nervosa (AN)

June 2011

Phase 3

View MoreCollapse

References

[1].Anagnostopoulou V, et al. Differential effects of
dehydroepiandrosterone and testosterone in prostate and colon cancer
cell apoptosis: the role of nerve growth factor (NGF) receptors.
Endocrinology. 2013
Jul;154(7):2446-56.

[2].McNelis JC, et al. Dehydroepiandrosterone exerts
anti-glucocorticoid action on human preadipocyte proliferation,
differentiation and glucose uptake. Am J Physiol Endocrinol Metab. 2013
Nov
1;305(9):E1134-44.

[3].Catalina F, et al. Decrease of core body temperature in mice by
dehydroepiandrosterone. Exp Biol Med (Maywood). 2002
Jun;227(6):382-8.

---